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. 2020 Apr 30;18:45. doi: 10.1186/s12915-020-00780-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Detection of human MIR941 following 2 weeks post AAV1 injection into the striatum of male mice. An AAV1 viral vector, encoding human pre-MIR941-1 (MIR941), was injected into a single striatal hemisphere of three male mice. Following sacrifice after 2 weeks post-injection, MIR941 was detected, using gene-specific locked nucleic acid (LNA) quantitative PCR (qPCR), in the injection site, lymph node and vas deferens/epididymis (V/E). Human embryonic kidney (HEK) 293 cells were used as a positive control for MIR941 while a no template control served as a negative control. *Samples that were positive on the gel (single band of expected size) and were supported by melt-curve analysis (Additional file 5: Supplementary Information)