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. 2020 Apr 30;18:45. doi: 10.1186/s12915-020-00780-w

Fig. 3.

Fig. 3

Sequencing and quantitation of ‘positive’ MIR941 LNA qPCR bands. a, b For example, we show that for Male 1 (Fig. 2), all bands that were considered ‘positive’ for gel size and melt-curve analysis were also positive for human MIR941 following cloning and Sanger sequencing. c Quantitative analysis using UniSp6 spike-in controls demonstrate that the amount of MIR941 found to be positive outside of the brain is ~ 1–2% of what is seen in the striatal injection sites