Fig. 5.

Sequencing and quantitation of ‘positive’ MIR941 LNA qPCR bands. a, b For example, we show that for Male 4 (Fig. 4), all bands that were considered ‘positive’ for gel size and melt-curve analysis were also positive for human MIR941 following cloning and Sanger sequencing. c Quantitative analysis using UniSp6 spike-in controls demonstrate that the amount of MIR941 found to be positive outside of the brain is ~ 1–8% of what is seen in the striatal injection sites