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. 2020 Apr 29;20:104. doi: 10.1186/s12866-020-01796-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Phosphotransfer between LiaS and LiaR. Phosphorylated LiaS at 4 μM was incubated with 20 μM LiaR in PB at room temperature. The reaction was quenched at various time intervals and samples were analyzed by 15% SDS-PAGE. Gels were exposed to a phosphor screen (top panel) and imaged using a Typhoon Trio⁺ Imager (GE Healthcare), followed by coomassie staining (bottom panel)