Table 7.
Primers used in the qRT-PCR analysis
| Gene | Identification | Sequence 5′ - > 3’ | GenBank Acession Number | |
|---|---|---|---|---|
| Ubiquitin | Reference gene | F | AAGGCCAAGATCCAGGACAA | GRMZM2G027378_T01 |
| R | TTGCTTTCCAGCGAAGATGA | |||
| SAD | Putative stearoyl-ACP desaturase | F | GGA TTT CCT CCC TGA CCC A | KU949326 |
| R | GTC CAT GCC CTC GTC CAA A | |||
| ZmMPK5 | Mitogen Activated Protein Kinase | F | ACTGATGGACCGCAAACC | AB016802 |
| R | GGGTGACG AGGAAGTTGG | |||
| SOD | Superoxide Dismutase/Antioxidant | F | TGGAGCACCAGAAGATGA | X17565 |
| R | CTCGTGTCC ACCCTTTCC | |||
| APX | Ascorbate Peroxidase/Antioxidant | F | TGAGCGACCAGGACATTG | EU969033 |
| R | GAGGGCTTTGTCA CTTGGT |
(F) sequence of the forward primer and (R) sequence of the reverse primer