Fig. 5.

Transcriptional regulation of microtubule organisation by GA₃ and ABA. Relative expression of TUA6 (a), TUB3 (b), TUB4 (c), TUB5 (d), TUB6 (e), MAP65-1 (f), MAP65-2 (g) and EB1a (h) genes in dormant and non-dormant Arabidopsis seeds. HOI, hours of imbibition at 25 °C in the dark; DOS, days of stratification at 4 °C in the dark. 0 HOI corresponds to dry dormant seeds and is used as a reference condition. Dormant seeds (d) were imbibed at 25 °C in the presence of gibberellic acid (GA₃) for 6, 24 and 48 h (6 HOGA₃, 24 HOGA₃ and 48 HOGA₃, respectively). Stratified non-dormant seeds (ND) were imbibed at 25 °C on water (0 HOABA) and in the presence of abscisic acid (ABA) for 6, 24 and 48 h (6 HOABA, 24 HOABA and 48 HOABA, respectively). Different letters indicate significant differences among samples (one-way ANOVA). Values are the means ± s.e. of 3 independent biological replicates with 3 technical replicates each