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AMPKα activation by emodin. HepG2 cells were incubated in serum free media for 12 h, and then treated with 30 μM Emo for indicated time periods. Western blotting analysis of p-ACC, p-AMPKα and β-actin and band intensity of p-ACC (**p < 0.01 between vehicle and Emo-treated cells). Western blotting analysis was performed with HepG2 (A), SK-Hep-1 (B) and Huh-7 cells (C). (D) Expression of p-LKB1 in HepG2 cells. (E) Compare of LKB1-deficient Hela cells and HepG2 cells. Emo: emodin.
