Figure 4.
A genetic complementation study confirms that a shared ADD3 mutation causes renal vascular dysfunction in FHH and MNS rats. (A) One copy of ADD3 WT K572 allele in an F1 cross of FHH and FHH.1BN congenic (FHH × 1BN) rats restored the Af-art myogenic response (left panel) and RBF autoregulation (right panel) relative to FHH rats. (B) Two copies of ADD3 mutant Q572 allele in an F1 cross of FHH and MNS rat (FHH × MNS) abolished the Af-art myogenic response (left panel) and RBF autoregulation (right panel). Numbers in parentheses indicate the number of animals studied. *Significant difference compared with the corresponding value in the WT strains; †significant difference from the corresponding value in Sprague Dawley rats.