| Antibodies |
| Rabbit anti-CRAMP |
University of California San Diego |
Dr. Richard Gallo |
| Rabbit anti-LL37 |
University of California San Diego |
Dr. Richard Gallo |
| goat anti-COLIV |
Abcam |
ab769 |
| Mouse anti-FABP4 |
Abcam |
ab188387 |
| Rabbit anti-Phospho-SMAD2, 3 |
Cell Signaling |
8828S |
| Rabbit anti-SMAD2,3 |
Abcam |
ab202445 |
| rabbit anti-phospho-AKT antibody |
Cell Signaling |
4060S |
| mouse anti-AKT |
Cell Signaling |
4691S |
| anti-mouse CD16/32 |
eBioscience |
14016185 |
| PECy7 anti-CD45 |
BioLegend |
147704 |
| PerCP-Cy5.5 anti-CD31 |
BioLegend |
102522 |
| PE anti-THY1 |
BioLegend |
105308 |
| APC anti-PDGFRA |
eBioscience |
17140181 |
| BV605 anti-Sca1 |
BioLegend |
108133 |
| AF488 anti-SMA |
eBioscience |
53976082 |
| APC anti-CD4 |
Biolegend |
100516 |
| BV510 anti-CD8a |
Biolegend |
100752 |
| APC-Cy7 anti-TCRβ |
Biolegend |
109220 |
| PE anti-TCRδγ |
eBioscience |
12-5711-82 |
| PerCP-Cy5.5 anti-CD45.2 |
Biolegend |
109828 |
| PECy7 anti-CD11B |
Biolegend |
101216 |
| FITC anti-Ly6G |
eBioscience |
11593182 |
| PE anti-F4/80 |
eBioscience |
56532182 |
| APC anti-CD11C |
Biolegend |
117310 |
| AF700 anti-MHCII |
eBioscience |
56532182 |
| zombie violet viability dye |
Biolegend |
423114 |
| Bodipy |
ThermoFisher Scientific |
D3922 |
| Bacterial and Virus Strains |
|
S. aureus USA300 |
University of California San Diego |
Dr. Victor Nizet |
|
S. aureus SA113 |
University of California San Diego |
Dr. Victor Nizet |
| Biological Samples |
|
|
| Mouse skin, spleen, blood samples |
University of California San Diego, Animal Facility |
N/A |
| Neonatal human skin samples |
International Institute for Advancement of Medicine (IIAM) |
N/A |
| Adult human skin samples |
University of California San Diego, Dermatology clinics |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| SB431542 |
Selleckchem |
S1067 |
| SIS3 |
Selleckchem |
S7959 |
| Wortmannin |
EMD Millipore |
9951 |
| 3-Isobutyl-1-methylxanthine (IBMX) |
SIGMA-ALDRICH |
I5879 |
| Indomethacin |
SIGMA-ALDRICH |
I8280 |
| Dexamethasone |
SIGMA-ALDRICH |
D4902 |
| Insulin |
SIGMA-ALDRICH |
91077C |
| DMEM, high glucose |
Thermo Fisher Scientific (Life Technologies) |
11965118 |
| Antibiotic-Antimycotic |
Thermo Fisher Scientific (Life Technologies) |
15240062 |
| Glutamax |
Thermo Fisher Scientific (Life Technologies) |
35050-061 |
| Recombinant mouse TGFβ1 |
R&D Systems |
7666-MB-005 |
| Recombinant mouse TGFβ2 |
R&D Systems |
7346-B2-005 |
| PureLink RNA Mini Kit |
Thermo Fisher Scientific (Life Technologies) |
12183025 |
| Collagenase D from Clostridium histolyticum |
Roche |
11088882001 |
| Deoxyribonuclease I from bovine pancreas,Type IV, lyophilized powder |
SIGMA-ALDRICH |
D5025-150KU |
| RNase-free DNase Set |
Qiagen |
79254 |
| iScript cDNA synthesis kit |
Bio-Rad |
1725038 |
| 2x SYBR Green qPCR Master Mix |
Bimake |
B21202 |
| Super Script II Reverse Transcriptase |
Invitrogen |
18064014 |
| KAPA HiFi Hotstart ReadyMix |
Kapa Biosystems |
KK2601 |
| AMPure XP beads |
Beckman Coulter |
A63881 |
| Critical Commercial Assays |
| Gomori Trichrome green collagen staining kit |
ThermoFisher Scientific |
87021 |
| Hydroxyproline assay kit |
BioVision |
K555-100 |
| Mouse TGF-beta 1 ELISA |
R&D Systems |
DY7346-05 |
| Mouse TGF-beta 2 ELISA |
R&D Systems |
DY7346-05 |
| Intracellular Fixation & Permeabilization Buffer Set |
eBioscience |
88-8824-00 |
| Nextera DNA Sample Preparation kit |
Illumina |
FC-121-1030 |
| NextSeq 500 High Output Kit v2 – 75 cycles |
Illumina |
FC-404-2005 |
| Deposited Data |
| RNA-seq data of cultured E14 ∼ 2 month dermal fibroblasts |
NCBI Gene Expression Omnibus (GEO) |
GEO accession #: GSE121460
|
| Experimental Models: Cell Lines |
| Primary mouse or human dermal fibroblasts |
This study |
Isolated from mouse or human skin dermis |
| Experimental Models: Organisms/Strains |
|
Campflox/flox mice |
This study |
Dr. Richard Gallo |
|
Pdgfra-cre mice |
Jackson laboratory |
013148 |
|
Adipoq-cre mice |
Jackson laboratory |
028020 |
| Tgfbr2flox/flox |
Jackson laboratory |
012603 |
| Oligonucleotides |
| Mouse primers for RTqPCR |
Integrated DNA Technologies (IDT) |
See Table S1 for primer sequences |
| Human primers for RTqPCR |
Integrated DNA Technologies (IDT) |
See Table S2 for primer sequences |
| Software and Algorithms |
| GraphPad Prism |
GraphPad Software, Inc. |
|
| FlowJo V10 |
FlowJo LLC |
|
| Ingenuity pathway analysis |
Qiagen |
|
| Bowtie |
Langmead et al. 2009 |
http://bowtie-bio.sourceforge.net/manual.shtml |
| RSEM |
Li and Dewey, 2011 |
https://github.com/deweylab/RSEM |
| Next maSigPro |
Nueda et al. 2014 |
https://www.bioconductor.org/packages/release/bioc/html/maSigPro.html |
