FIGURE 1.

Marseillevirus gene expression classes. (A) Heat map of Marseillevirus gene expression profiles. Rows correspond to all 457 putative genes and columns to the 9 infection time points (0 h corresponds to 30 min of infection due to virus adsorption period). Expression profiles are clustered using hierarchical clustering. A dendrogram of the clustering is shown on the left. (B) Heat map of the same expression profiles partitioned into three main classes, “early” (top), “intermediate” (center), and “late” (bottom), with the k-means clustering algorithm. Expression levels are displayed from green (low expression) to red (high expression). Gray lines indicate absence of data for the gene at a particular infection time point. Both clustering methods were performed by applying Euclidean distance as a similarity metric. Among the 457 MRSV-encoded genes, 83 (18%) are expressed early, 218 (48%) are intermediate genes, and 156 (34%) are late genes. The function of each gene related to their temporal expression pattern can be found in Supplementary Data Sheet S1.