Figure 2. Uricase-deficient rat identified by Western blot (A) and RT-PCR (B).

Uricase protein from wild-type (WT) rat is about 35 kDa and the encoded mRNA results in a PCR product of about 500 bp. As controls, β-actin (42 kDa) was both positive in Uox^−/− and WT rats, and so was GAPDH (glyceraldehyde 3 phosphate dehydrogenase) (149 bp) in RT-PCR. However, Uox^−/− rats express no normal uricase both at protein level and mRNA level.