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. Author manuscript; available in PMC: 2020 Aug 14.
Published in final edited form as: J Am Chem Soc. 2019 Aug 5;141(32):12648–12656. doi: 10.1021/jacs.9b04615

Figure 4.

Figure 4.

Efficacy of VIQKI-PEG24-chol against RSV infection in human airway epithelial (HAE) cultures. HAE cultures were infected by applying 100 μl of EpiAirway medium containing 4,000 PFU of RSV to the apical surface for 90 min at 37 °C. The cultures were inoculated with a single dose of VIQKI–PEG24–Chol (10 μM, blue) or untreated control (black). The medium containing the inoculum was removed, cultures were placed at 37 °C and fed each day with 0.9 ml medium via the basolateral surface. Viruses were harvested by adding 200 μl medium per well to the HAE cultures’ apical surface and allowed to equilibrate for 30 min at 37 °C. The suspension was then collected, and viral titers were determined. Viral collection was performed sequentially with the same wells of cells on each day post infection.