Table 1.
Virus real‐time PCR Taqman assays
| Primer or probe | Oligonucleotide sequences (5′‐3′) and fluorescent labels | Target |
|---|---|---|
| Rhinovirus (HRV) | ||
| Primer RHFWD | GCACTTCTGTTTCCCC | 5′ UTR |
| Primer RHRVS | GGCAGCCACGCAGGCT | |
| Probe RHP1 | FAM‐AGCCTCATCTGCCAGGTCTA‐TAMRA | |
| Probe RHP2 | FAM‐AGCCTCATCGACCAAACTA‐TAMRA | |
| Enterovirus (EV) | ||
| Primer EVFWD | TCCTCCGGCCCCTGA | 5′ UTR |
| Primer EVRVS | RATTGTCACCATAAGCAGCCA | |
| Probe EVP | FAM‐CGGAACCGACTACTTTGGGTGWCCGT‐TAMRA | |
| Respiratory syncytial virus type A | ||
| Primer RSVAFWD | AGATCAACTTCTGTCATCCAGCAA | N gene |
| Primer RSVARVS | GCACATCATAATTAGGAGTATCAAT | |
| Probe RSVAP | FAM‐CACCATCCAACGGAGCACAGGAGAT‐TAMRA | |
| Respiratory syncytial virus type B | ||
| Primer RSVBFWD | AAGATGCAAATCATAAATTCACAGGA | N gene |
| Primer RSVBRVS | TGATATCCAGCATATTTAAGTATCTTTATAGTG | |
| Probe RSVBP | FAM‐AGGTATGTTATATGCTATGTCCAGGTTAGGAAGGGAA‐TAMRA | |
| Metapneumovirus | ||
| Primer ALTNFWD | CAACAACATAATGCTAGGACATGTATC | nucleoprotein gene |
| Primer ALTNRVS | CCGAGAACAACACTAGCAAAGTTG | |
| Probe ALTNP | FAM‐TGGTGCGAGAAATGGGTCCTGAATCTGG‐TAMRA | |
| Primer NLNFWD | CATATAAGCATGCTATATTAAAAGAGTCTC | |
| Primer NLNRVS | CCTATTTCTGCAGCATATTTGTAATCAG | |
| Probe NLNP | FAM‐TGYAATGATGAGGGTGTCACTGCGGTTG‐TAMRA | |
| Non‐SARS coronavirus | ||
| Primer N229EFWD | CAGTCAAATGGGCTGATGCA | N gene |
| Primer N229ERVS | AAAGGGCTATAAAGAGAATAAGGTATTCT | |
| Probe N229EP | FAM‐CCCTGACGACCACGTTGTGGTTCA‐TAMRA | |
| Primer NOC43FWD | CGATGAGGCTATTCCGACTAGGT | |
| Primer NOC43RVS | CCTTCCTGAGCCTTCAATATAGTAACC | |
| Probe NOC43P | FAM‐TCCGCCTGGCACGGTACTCCCT‐TAMRA |
FAM, 6‐carboxyfluorescein; TAMRA, 6‐carboxy‐tetramethyl‐rhodamine; degenerate primers: W = A/T, R = A/G, Y = C/T.