KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat polyclonal anti-IL-1β | R&D Systems | Cat#AF-401-NA; RRID:AB_416684 |
| Rabbit polyclonal anti-IL-1β | Genetex | Cat#GTX74034; RRID:AB_378141 |
| Mouse monoclonal anti-Caspase-1 (clone Casper-1) | Adipogen | Cat#AG-20B-0042-C100; RRID:AB_2755041 |
| Rat monoclonal anti-Caspase-8 (clone 1G12) | Enzo Life Sciences | Cat#ALX-804-447-C100; RRID:AB_2050952 |
| Rabbit monoclonal anti-cleaved Caspase-8 (clone D5B2) | Cell Signaling Technology | Cat#8592; RRID:AB_10891784 |
| Rabbit polyclonal anti-Caspase-3 | Cell Signaling Technology | Cat#9662; RRID:AB_331439 |
| Rabbit polyclonal anti-cleaved Caspase-3 | Cell Signaling Technology | Cat#9661; RRID:AB_2341188 |
| Rabbit monoclonal anti-FLIP (clone D5J1E) | Cell Signaling Technology | Cat#56343; RRID:AB_2799508 |
| Rabbit polyclonal anti-Caspase-6 | Cell Signaling Technology | Cat#9762; RRID:AB_10829240 |
| Rabbit monoclonal anti-Caspase-7 (clone D2Q3L) | Cell Signaling Technology | Cat#12827; RRID:AB_2687912 |
| Mouse monoclonal anti-Caspase-9 (clone C9) | Cell Signaling Technology | Cat#9508; RRID:AB_2068620 |
| Rabbit monoclonal anti-GSDMD (clone EPR19828) | Abeam | Cat#ab209845; RRID:AB_2783550 |
| Rabbit monoclonal anti-DFNA5/GSDME (clone EPR19859) | Abeam | Cat#ab215191; RRID:AB_2737000 |
| Mouse monoclonal anti-actin (clone AC-15) | Sigma-Aldrich | Cat#A5441; RRID:AB_476744 |
| Anti-mouse CD11c (clone N418) | Thermo Fisher | Cat#MCD11c05; RRID:AB_10373550 |
| Anti-mouse I-A/I-E (clone M5/114.15.2) | Thermo Fisher | Cat#46-5321-82; RRID:AB_1834439 |
| Anti-mouse CD11b (clone M1/70) | Thermo Fisher | Cat#11-0112-82; RRID:AB_464935 |
| Anti-mouse CD115 (clone AFS98) | Thermo Fisher | Cat#17-1152-82; RRID:AB_1210789 |
| Anti-mouse CD135 (clone A2F10) | Thermo Fisher | Cat#12-1351-82; RRID:AB_465859 |
| Anti-mouse MerTK (clone DS5MMER) | Thermo Fisher | Cat#25-5751-82; RRID:AB_2573466 |
| Anti-mouse TCR β chain (clone H57-597) | Biolegend | Cat#109229; RRID:AB_10933263 |
| Anti-mouse CD3 (clone 145-2C11) | Biolegend | Cat#100340; RRID:AB_11149115 |
| Anti-mouse FasL (clone MFL3) | Biolegend | Cat#106606; RRID:AB_313279 |
| Anti-phycoerythrin antibody (clone PE001) | Biolegend | Cat#408102; RRID:AB_2168924 |
| Anti-mouse FasL blocking antibody (clone MFL3) | Biolegend | Cat#106612; RRID:AB_2813954 |
| Armenian Hamster IgG isotype control antibody | Biolegend | Cat#400940; RRID:AB_11203529 |
| Anti-human FasL blocking antibody (clone NOK-1) | Biolegend | Cat#306415; RRID:AB_2810458 |
| Mouse IgG1, κ isotype control antibody (clone MOPC-21) | Biolegend | Cat#400165; RRID:AB_11150399 |
| Biotinylated polyclonal goat anti-mouse IL-1β | R&D Systems | Cat#BAF401; RRID:AB_356450 |
| Goat anti-mouse IgG (H+L) | Jackson ImmunoResearch | Cat#115-035-146; RRID:AB_2307392 |
| Donkey anti-Rabbit IgG (H+L) | Jackson ImmunoResearch | Cat#711-035-152; RRID:AB_10015282 |
| Donkey anti-Rat IgG (H+L) | Jackson ImmunoResearch | Cat#712-035-150; RRID:AB_2340638 |
| Bovine anti-Goat IgG (H+L) | Jackson ImmunoResearch | Cat#805-035-180; RRID:AB_2340874 |
| Rat anti-mouse CD16/CD32 (clone 93) | Thermo Fisher | Cat#16-0161-86; RRID:AB_468900 |
| Bacterial and Virus Strains | ||
| Bacteroidis fragilis (strain NCTC 9343) | Laboratory of Dr. Laurie Comstock | ATCC 25285 |
| Chlamydia trachomatis (strain D/UW-3/Cx) | Laboratory of Dr. Michael Starnbach | ATCC VR-85 |
| Biological Samples | ||
| Human peripheral blood mononuclear cells | Brigham and Women’s Hospital Kraft Family Blood Donor Center | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant, multimeric FasL (MegaFasL) | Adipogen | Cat#AG-40B-0130-C010 |
| Pam3CSK4 | Invivogen | Cat#tlrl-pms |
| Nigericin | Invivogen | Cat#tlrl-nig |
| MSU Crystals | Invivogen | Cat#tlrl-msu |
| Alpha Galactosylceramide | Dr. Gurdyal Besra | N/A |
| Recombinant murine GM-CSF | Peprotech | Cat#315-03 |
| Recombinant human GM-CSF | Peprotech | Cat#300-03 |
| Recombinant murine IL-2 | Peprotech | Cat#212-12 |
| Recombinant murine IL-7 | Peprotech | Cat#217-17 |
| Recombinant human IL-2 | Peprotech | Cat#200-02 |
| Mouse PBS-57 CD1d-APC tetramer | NIH Tetramer Core Facility | N/A |
| Collagenase, type 4 | Worthington Biochemical | Cat#LS004186 |
| DNase 1 | Sigma-Aldrich | Cat#DN25 |
| ACK lysing buffer | Thermo Fisher | Cat#A1049201 |
| SYTOX Green | Thermo Fisher | Cat#S34860 |
| YOYO-1 | Thermo Fisher | Cat#Y3601 |
| Annexin V Alexa Fluor 488 | Thermo Fisher | Cat#A13201 |
| Annexin V Alexa Fluor 647 | Thermo Fisher | Cat#A23204 |
| Annexin V APC | Biolegend | Cat#640941 |
| SiR-DNA | Cytoskeleton, Inc | Cat#CY-SC007 |
| FluoroBrite DMEM | Thermo Fisher | Cat#A1896702 |
| Opti-MEM | Thermo Fisher | Cat#11058021 |
| Poly-D-Lysine | Sigma-Aldrich | Cat#A-003-E |
| Glycine | Sigma-Aldrich | Cat#G7126 |
| CellTrace Violet | Thermo Fisher | Cat#C34557 |
| Zombie Green Fixable Viability Dye | Biolegend | Cat#423112 |
| Dynabeads MyOne Streptavidin T1 | Thermo Fisher | Cat#65601 |
| Trichloroacetic acid | Sigma-Aldrich | Cat#91228 |
| ActinGreen-488 | Thermo Fisher | Cat#R37110 |
| Hoechst 33342 | Thermo Fisher | Cat#H3570 |
| SlowFade Diamond Antifade Mountant | Thermo Fisher | Cat#S36963 |
| Brain Heart Infusion broth | Anaerobe Systems | Cat#AS-872 |
| Ficoll-Paque PLUS | GE Lifesciences | Cat#17144003 |
| Critical Commercial Assays | ||
| Mouse IL-1β ELISA kit | Thermo Fisher | Cat#88-7013-77; RRID:AB_2574944 |
| Mouse IFNγ ELISA kit | Thermo Fisher | Cat#88-7314-77; RRID:AB_2575068 |
| Human IL-1β ELISA kit | Thermo Fisher | Cat#88-7261-88; RRID:AB_2575054 |
| Pierce™ LDH cytotoxicity assay | Thermo Fisher | Cat#88954 |
| Anti-mouse CD11c microbeads, ultrapure | Miltenyi Biotec | Cat#130-108-338 |
| Mouse Pan T Cell Isolation Kit II | Miltenyi Biotec | Cat#130-095-130 |
| Anti-APC microbeads | Miltenyi Biotec | Cat#130-090-855 |
| Anti-human CD14 microbeads, ultrapure | Miltenyi Biotec | Cat#130-118-906 |
| Pierce BCA Protein Assay | Thermo Fisher | Cat#23227 |
| eBioscience Intracellular Fixation & Permeabilization Buffer Set | Thermo Fisher | Cat#88-8824-00 |
| Click-iT Plus TUNEL Assay, Alexa Fluor 647 dye | ThermoFisher | Cat#C10619 |
| Deposited Data | ||
| ImmGen Consortium | Heng et al., 2008 | https://www.immgen.org |
| Experimental Models: Cell Lines | ||
| Primary murine splenic iNKT cell line | This paper | N/A |
| Primary human iNKT cell clone J24L.17 | Brigl et al., 2006 | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Fas−/−: B6.MRL-Faslpr/J | Jackson Laboratories | JAX stock #000482 |
| Mouse: Caspase1/11−/−: B6N.129S2-Casp1tm1Flv/J | Jackson Laboratories | JAX stock #016621 |
| Mouse: CD1d−/− | Exley et al., 2003 | N/A |
| Mouse: Caspase-8DA/DA | Philip et al., 2016 | N/A |
| Mouse: Gsdmd−/− | Kambara et al., 2018 | N/A |
| Mouse: Ripk3−/− | Newton et al., 2004 | N/A |
| Mouse: Mlkl−/− | Murphy et al., 2013 | N/A |
| Mouse: Ripk3−/−Casp8−/− | Kaiser et al., 2011 | N/A |
| Mouse: Gsdme−/−: C57BL/6N-Gsdmeem1Fsha/J | Jackson Laboratories | JAX stock #032411 |
| Mouse: Gsdmd−/−Gsdme−/− | Chen et al., 2019 | N/A |
| Mouse: Asc−/− | Ozören et al., 2006 | N/A |
| Mouse: WT: C57BL/6NJ (control for Gsdme−/− mice) | Jackson Laboratories | JAX stock #005304 |
| Mouse: WT: C57BL/6 | Jackson Laboratories | JAX stock #000664 |
| Mouse: Nlrp3−/−: B6.129S6-Nlrp3tm1Bhk/J | Jackson Laboratories | JAX stock #021302 |
| Software and Algorithms | ||
| FlowJo (v10.5.3) | BD Biosciences | https://www.flowjo.com/ |
| R (V3.3.2) | R Foundation for Statistical Computing | http://www.R-project.org |
| Fiji / ImageJ version 2.0.0 | Fiji contributors / ImageJ developers | https://imagej.net/Fiji |
| Zen (v2.3) | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| MetaXpress (v6.0) | Molecular Devices | https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metaxpress |
| GraphPad Prism 7 | GraphPad Software | https://www.graphpad.com/ |
| Multiplot Studio v2 | GenePattern, Broad Institute | https://www.genepattern.org/ |
| Other | ||
| 96-well plate | No. 1.5 Coverslip | 5 mm Glass Diameter | Poly-D-Lysine Coated | MatTek Corporation | Cat#P96GC-1.5-5-F |
| Greiner Bio-One SensoPlate 96-Well Glass Bottom Microplates | Greiner Bio-One | Cat#655892 |