Figure 3. The KCC2^E/+ P15 but not P30 mice show an altered glutamate/GABA balance.

A) Representative whole cell recordings of brain slices spontaneous post-synaptic currents (holding potential =−45mV) in 2 week-old KCC2^+/+ and KCC2^E/+ CA3 pyramidal neurons. Scale values: 500 ms, 50 pA. B to F) Boxplots of frequencies (B,C) and amplitudes (E,F) of the spontaneous glutamatergic (B and E) and GABAergic (C and F) post-synaptic currents, and their ratios (D), recorded from 2 and 4 week-old KCC2^+/+ and KCC2^E/+ CA3 pyramidal neurons. Numbers in parenthesis indicate the number of cells recorded and mice used. *P<0.05, ***P<0.001, Mann-Whitney test (see table S3 for details on statistical analyses).