Table 1. Proteins co-purifying with His-Spf1.
Protein Mr, intracellular location, median abundance and function was obtained from the Saccharomyces cerevisiae Genome Database (SGD). Location: MIT, mitochondria; C, cytosol; N, nucleus; PM, plasma membrane; V, vacuole. Poly-His refers to the presence of a continuous series of at least 6xHis in the primary sequence.
| PROTEIN | Mr (kDa) | LOCATION | ABUNDANCE (molecules/cell) | POLY HIS | FUNCTION |
|---|---|---|---|---|---|
| POR1 Mitochondrial outer membrane porin 1 | 30 | MIT | 21873 ±16504 | no | Voltage-gated anion channel; involved in ion transport, cellular redox homeostasis |
| SRO9, RNA-binding protein | 48 | C, N | 26393 ± 9666 | no | RNA-binding protein involved in cytoplasmic translation |
| yCK2 Casein kinase I homolog2 | 62 | PM | 5636 ± 1685 | no | Morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing |
| PHO8 repressible alkaline phosphatase | 63 | V | 3505 ± 1618 | no | Phosphate metabolism, dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN |
| SSA1 Heat Shock protein | 70 | C,N,PM,V | 255901 ±159925 | no | ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; required for ubiquitin-dependent degradation of short-lived proteins |
| RTK1 Probable serine-threonine protein kinase | 70 | C | 3256 ± 1709 | yes | Ribosome biogenesis and tRNA synthetase-associated kinase phosphorylated by Cdc28 |
| SNF1 Carbon catabolite-derepressing protein kinase | 72 | C,N,V,M | 5622 ± 2394 | yes | AMP-activated protein kinase, required for glucose-repressed gene transcription, heat shock, sporulation, and peroxisome biogenesis |
| YGR266W uncharacterized protein | 81 | M, PM | 3230 ± 1379 | no | Unknown function |
| PMT4, dolichyl-phosphate-mannose protein mannosyltransferase | 88 | ER | 3728 ± 1570 | no | Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues |
| CHO2 Phosphatidylethanolamine N methyltransferase | 101 | ER | 7198 ± 3311 | no | Conversion of phosphatidylethanolamine to phosphatidylcholine |
| IST2 increased sodium tolerance protein 2 | 106 | ER | 4124 ± 1768 | yes | ER-plasma membrane tethering |