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. 2020 Apr 30;15(4):e0232536. doi: 10.1371/journal.pone.0232536

Fig 3. Effect of SDF1 and the CXCR4 inhibitor on in vitro sperm migration and motility.

Fig 3

(A) Sperm chemotaxis toward SDF1 was evaluated by a modified chemotaxis chamber technique. The chemotaxis chamber was separated into upper and lower chambers by a filter membrane with 8 μm pores. The lower chamber was filled with a sperm suspension, and the upper chamber was filled with medium containing SDF1 at final concentrations of 0 (Control), 0.1, 1 and 10 ng/ml. The chamber was incubated for 30 min, and the number of sperm in the upper chamber was calculated. White bars show the numbers for the control group, and black bars show those for the AMD3100 group. Data are shown as the mean ± SE (*p<0.05, **p<0.01). n = 3. (B) Comparison of the motility parameters of sperm with different treatments. Each sperm sample was evaluated using a CASA system after 1, 30, and 60 min. Top: Motile (%); Middle: Straight line velocity (μm/sec); Bottom: Curvilinear velocity (μm/sec). Data are shown as the mean ± SE. n = 4.