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. 2020 Apr 30;15(4):e0232536. doi: 10.1371/journal.pone.0232536

Fig 6. Effect of the CXCR4 inhibitor on the in vitro fertilization rate.

Fig 6

The fertilization rates of conventional IVF and 12-well dish IVF in the presence or absence of AMD3100. In conventional IVF, groups of in vitro-matured COCs (≤50) were introduced into 200 μl microdrops of a sperm suspension in a 35-mm culture dish and cocultured for 6 h. In the 12-well dish IVF, a single mature COC and sperm suspension were placed in the specified wells of the dishes and cocultured for 6 h. Then, 20 μM AMD3100 was added to the sperm suspension to investigate its effect on fertilization rates. Eighteen hours after coincubation, denuded oocytes were fixed with acetic acid/ethanol. The pronuclei of each oocyte were observed, and an oocyte with two or more pronuclei was regarded as a fertilized oocyte. White bars show the numbers for the control group, and black bars show those for the AMD3100 group. The numbers above each bar indicate the number of fertilized oocytes per total number of evaluated oocytes.