Table 1. List of 22 tested TSs and their activity.
| Gene ID | Source | Products | ||||
|---|---|---|---|---|---|---|
| GPP (C10) | FPP (C15) | GGPP(C20) | ||||
| 1 | AHY47823.1 | RrNerS | Rubrobacter radiotolerans | β-Myrcene‡, Ocimene†, R/S-Linalool†, Geraniol‡ | (±)-Nerolidol#, α/β-Farnesene‡, Farnesol‡ | NT |
| 2 | AHY45426.1 | RrBerS | Rubrobacter radiotolerans | β-Myrcene‡, Linalool#, trans-Geraniol‡ | α-Bergamotene#, (±)-trans Farnesene‡, (±)-trans Nerolidol‡, Farnesol‡ | NT |
| 3 | KYF56472.1 | ScAroS | Sorangium cellulosum | cis and trans- Ocimene‡, β-Pinene‡, R/S-Linalool‡ | Aromandendrene# | NT |
| 4 | KFG92939.1 | BpLonS | Burkholderia paludis | Geraniol‡ | (±)-Cadinene#, Longiborneol# | NT |
| 5 | AHH94051.1 | KaGerS | Kutzneria albida DSM 43870 | ND | Germacrene D# Germacradienol#, | ND |
| 6 | KPH97530.1 | AbAcoS | Actinobacteria bacterium OV450 | ND | Copaene†, Acora-3,7(14)-diene† | NT |
| 7 | ADU09376.1 | MiGerS | Micromonospora sp. L5 | ND | Germacrene A#, 1(10),4,11-Germacratriene# | NT |
| 8 | CAN96536.1 | ScEpiS | Sorangium cellulosum So ce56 [49] | cis and trans- Ocimene#, R/S Linalool#, β-Pinene | 10-epi-α/β-Cubinene#, β-Copaene #, Cadina-3,5-diene#, Cubebol#, α/γ/ δ-Cadinene#, 1,10-di-epi-Cubebol#, Cubenol# | ND |
| 9 | CAB41566.1 | ScGeoS | Streptomyces coelicolor A3(2) | β-Myrcene†, β-Ocimene†, Linalool†, Geraniol† | Geosmin†, Germacradienol#, Germacrene D# | NT |
| 10 | ACY98649.1 | TcCubS | Thermomonospora curvata DSM 43183 | ND | Cubebol‡ | ND |
| 11 | EXG82599.1 | CaCubS | Cryptosporangium arvum DSM 44712 | ND | Cubebol‡ | ND |
| 12 | ELQ82238.1 | SrGuaS | Streptomyces rimosus | ND | Guaia-1(10),11-diene‡, (+)-γ-Gurjunene‡ | ND |
| 13 | ABU58787.1 | RcDTPS | Roseiflexus castenholzii DSM 13941 | ND | ND | 2–3 unidentified compounds‡ |
| 14 | EJL71407.1 | CsDTPS | Chryseobacterium sp. CF314 | ND | ND | 2 unidentified compounds‡ |
| 15 | KFE96946.1 | ClDTPS | Chryseobacterium. Luteum | ND | ND | 2–3 unidentified compounds‡ |
| 16 | CFM47198.1 | BpTPS | Burkholderia pseudomallei | ND | ND | ND |
| 17 | KYF59190.1 | ScTPS1 | Sporangium cellulosum | ND | ND | ND |
| 18 | KFF23441.1 | CvTPS | Chryseobacterium vrystaatense | ND | ND | ND |
| 19 | KJK55818.1 | SsTPS | Saccharothrix sp. ST-888 | ND | ND | ND |
| 20 | KYF75876.1 | ScTPS2 | Sporangium cellulosum | ND | ND | ND |
| 21 | EST32145.1 | SniTPS | Streptomyces niveus NCIMB 11891 | ND | ND | ND |
| 22 | KIZ18902.1 | SnaTPS | Streptomyces natalensis ATCC 27448 | ND | ND | ND |
The products obtained by in vitro assay with purified TSs upon incubation with GPP and FPP or in vivo cultivation in E. coli are listed.
†–produced in in vitro conditions
‡–produced in in vivo conditions
#—produced in in vitro and in vivo conditions; ND–not detected, NT–not tested