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. 2020 Apr 30;5(3):440–453. doi: 10.1093/jalm/jfaa019

Table 1.

Primer sequences used for PCR amplification, Sanger sequencing and subsequent HRMA of the rpoB gene’s region known to determine rifampicin resistance (15).

Primer Sequence Annealing temperature(°C) Product size(bp) Nucleotide position Accession number
rpoB-F CGCGATCAAGGAGTTCTTC 65 118 2339 to 2357 L27989.1
rpob-R TGACAGACCGCCGGGCCC 2456 to 2439