Skip to main content
. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Mol Immunol. 2019 Dec 26;118:142–152. doi: 10.1016/j.molimm.2019.12.011

FIGURE 1. C4 and C3 fragments are deposited on HMEC-1 cells after exposure to anti-HLA antibody and normal human serum.

FIGURE 1.

HMEC cells were treated with W6/32 antibody and then exposed to normal human serum. Complement activation on the cells was then analyzed by flow cytometry after various periods of time. Data are plotted as mean fluorescence intensity (MFI) ± SEM. A) C4c was rapidly deposited on the cell surface (**P<0.01, ***P<0.001 vs control) but was significantly decreased by 60 minutes (#P<0.05 vs 60 minutes). B) C4d was also quickly deposited but remained on the cell surface with no significant change from 30 to 60 minutes (***P<0.001 vs control). C) Immunofluorescence microscopy confirmed deposition of C4d on the cell surface. Nuclei are stained with Dapi (blue). Original magnification x600. D) C3b/iC3b was detected on the cell surface at 15 minutes but was not statistically different than control by 60 minutes (*P<0.05, ***P<0.001 vs control). E) C3b/iC3b/C3dg was rapidly deposited and did not change significantly from 15 to 60 minutes (***P<0.001 vs control). C) Immunofluorescence microscopy confirmed deposition of C3d on the cell surface. Nuclei are stained with Dapi (blue). Original magnification x600.