Table 1. Quantitative summary of EAP experiments.

EAP	Movie number	Initiation	Stabilization	Initiation+Stabilization	Maturation	Biochemical measurements of CME
siRNA or mutant	${\mathit{n}}_{\mathit{s}\mathit{i}\mathit{C}\mathit{t}\mathit{r}\mathit{l}},$ ${\mathit{n}}_{\mathit{s}\mathit{i}\mathit{E}\mathit{A}\mathit{P}}$	CS initiation rate	CCP%	CCP rate	Median lifetime of CCP	TfRint (internal)	TfReff (internal/surface bound)
α-PIP2	19, 20	↑36%***	↓27%***	↑27%**	↓*	--	--
CALM	20, 19	↓38%***	↓30%***	↓67%***	↑25%***	↑21%***	↓64%***
epsin1	23, 22	→	↓30%***	↓24%**	→	↓37%***	→
Eps15	--, 23	↓21%***	↓31%***	↓46%***	↓12%**	→	↑22%**
Eps15R	--, 24	↓19%**	↓17%**	↓35%***	→	↓13%***	→
FCHO1	24, 24	→	→	→	↑10%*	↓30%***	→
FCHO2	--, 24	→	↓12%**	→	→	↓22%***	↓34%***
ITSN1	20, 19	→	↓33%***	↓30%**	→	↓22%***	↓9%*
ITSN2	--, 22	↓13%*	↓19%***	↓37%***	↑16%***	↓31%***	↓21%***
NECAP1	22, 21	→	↓26%***	↓39%***	↑20%*	→	↓24%**
NECAP2	--, 21	→	→	→	→	→	↓13%**
SNX9	24, 24	↓33%***	↓38%***	↓64%***	↑54%***	↑21%***	↓57%***

↑=increase; ↓=decrease; →=no significant change, p-value>0.05; *** p-value<0.001; ** p-value<0.01; * p-value<0.05 (statistical tests explained in Materials and methods). P-values and percentage changes are mean values obtained from 300 bootstraps comparing between KD conditions and bootstrapped siControl.