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. 2020 Feb 27;48(8):4147–4160. doi: 10.1093/nar/gkaa122

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — The acidic N-terminal ED-domain of dBigH1 perturbs RNApol II binding. (A) The normalized log₂ coverage ratio IP/input of Rpb3 in control mock-induced cells (left), dBigH1-expressing cells (center) and dBigH1ΔED-expressing cells (right) are presented as a function of the distance to TSS for up-regulated genes (red), down-regulated genes (blue) and non-DE genes (gray). (B) The relative Rpb3 occupancy with respect to control mock-induced cells was determined by ChIP-qPCR at the indicated genomic regions in dBigH1-expressing cells (black) and dBigH1ΔED-expressing cells (white) (N = 2; error bars are SD; Mixed linear model Benjamini-Hochberg adjusted P-values with respect to mock-induced cells are indicated). (C) As in B but for the promoter-proximal RNApol IIo^ser5 form at TSS of the indicated genes (N = 3 (dBigH1), 2 (dBigH1ΔED); error bars are SD; Mixed linear model Benjamini–Hochberg adjusted P-values with respect to mock-induced cells are indicated).