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. 2020 Mar 30;48(8):4405–4417. doi: 10.1093/nar/gkaa173

Figure 2.

Figure 2.

Steady-state expression of LeishIF4E-1, LeishIF4E-4, Leish4E-IP1, Leish4E-IP2 along the growth curve of promastigotes and axenic amastigotes. (A) Aliquots were collected daily (D1–D4: days 1–4) from L. amazonensis promastigotes and samples containing equal protein loads of cell lysates were resolved by SDS-PAGE. The gels were subjected to western blot analysis with antibodies directed against LeishIF4E-1 (4E1), LeishIF4E-4 (4E4), Leish4E-IP1 (IP1) and Leish4E-IP2 (IP2). LeishIF4A1(4A) was used as a loading control. The multiple bands observed for Leish4E-IP1 and Leish4E-IP2 could suggest that they are susceptible to break down. (B) As described in A, except with aliquots obtained from promastigote extracts from a day 2 culture and axenic amastigotes extracts (day 4 following differentiation) separated by SDS-PAGE. (C) Dot plot represents densitometry analysis (based on three replicate experiments) of the steady-state expression of LeishIF4E-1 (black circles in the left graph), LeishIF4E-4 (blue circles in the left graph), Leish4E-IP1 (red circles in the right graph) and Leish4E-IP2 (purple circles in the right graph).