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. 2020 Mar 30;48(8):4448–4462. doi: 10.1093/nar/gkaa201

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Requirement of C-terminal domains for mycobacterial topoisomerase I relaxation activity. (A) Assay of relaxation of negatively supercoiled plasmid DNA by wild-type or mutant MsmTOP1, MtbTOP1 truncated at the end of D5 (MsmTOP1-701t, MtbTOP1-704t), D6 (MsmTOP1-785t, MtbTOP1-786t), D7 (MsmTOP1-839t, MtbTOP1-840t) and D8 (MsmTOP1-909t, MtbTOP1-910t). (B) Summary of relaxation activity of the mutants with truncated C-terminal domains. (C) Complementation of E. coli AS17 with topA^ts chromosomal mutation for growth at 42°C by wild-type or mutant MtbTOP1 or MsmTOP1 produced by leaky expression from the T7 promoter in the 2O-T cloning vector.