Table 1.

Oligonucleotide substrates for crystallization and activity assays

	Length	Sequence
STS32	32	5′-CAGTGAGCGAGCTTCCGC↓TTGACATCCCAATA-3′
MTS2-25	25	5′-CAGTGAGCGAGCTTCCGC↓TTGACTT-3′
MTS3-14	14	5′-CTTCCGC↓TTGACAT-3′
MTS2-14	14	5′-CTTCCGC↓TTGACTT-3′
MTS2-13	13	5′-TTCCGC↓TTGACTT-3′

Cleavage position indicated by ↓ was mapped for MtbTOP1 in previous study (26).