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. 2020 Mar 17;48(8):4179–4194. doi: 10.1093/nar/gkaa146

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Fusion index of immortalized FSHD myotubes in the presence of berberine. Immortalized FSHD myotubes (A). FSHD-A5 or (C). FSHD-6 were treated with berberine at the second day of differentiation. Myosin heavy chain and nuclei were stained at the fourth day of differentiation with MF20 antibody and DAPI, respectively. Scale bar: 100 μm. Quantifying cell fusion index results for (B) FSHD-A5 and (D) FSHD-6, with nuclei counted in myotubes containing more than two nuclei and expressed as a percentage of the total nuclei present. Independent treatment at each concentration was performed, N = 6, *P < 0.05; **P < 0.01; ***P < 0.001, one -way ANOVA. Error bars represent S.E.M.