Table 1.
Primers list
| Target gene | Accession no. | Primer | Sequence (5′-3′) | Product size | |
|---|---|---|---|---|---|
| PCR primers | DUX4 | ENSG00000260596 | Forward | CCCAGGTACCAGCAGACC | 164 bp |
| Reverse | TCCAGGAGATGTAACTCTAATCCA | ||||
| B2M | NM_004048.2 | Forward | CTCTCTTTCTGGCCTGGAGG | 67 bp | |
| Reverse | TGCTGGATGACGTGAGTAAACC | ||||
| qPCR primers | DUX4 | ENSG00000260596 | Forward | CTCTGTGCCCTTGTTCTTC | 98 bp |
| Reverse | TCCAGGAGATGTAACTCTAATCCA | ||||
| Wfdc3 | ENSMUSG00000076434 | Forward | CTTCCATGTCAGGAGCTGTG | 134 bp | |
| Reverse | ACCAGGATTCTGGGACATTG | ||||
| Gapdh | ENSMUSG00000057666 | Forward | TCCATGACAACTTTGGCATTG | 103 bp | |
| Reverse | TCACGCCACAGCTTTCCA | ||||
| MBD3L2 | NM_144614.3 | Forward | CGTTCACCTCTTTTCCAAGC | 142 bp | |
| Reverse | AGTCTCATGGGGAGAGCAGA | ||||
| TRIM43 | NM_138800.1 | Forward | ACCCATCACTGGACTGGTGT | 100 bp | |
| Reverse | CACATCCTCAAAGAGCCTGA | ||||
| ZSCAN4 | NM_152677.2 | Forward | CTGGAGCAGTTTATGATTGG | 162 bp | |
| Reverse | AGCTTCCTGTCCCTGCATGT |