. 2020 Mar 18;48(8):4551–4561. doi: 10.1093/nar/gkaa170

Table 1.

ITC analysis on DNA binding of SATB1-CUTr1

DNA sequences^a	NaCl^b	K _A ^c	ΔG^c	ΔH^c	ΔS^c	Apparent stoichiometry
	(mM)	(10⁶ M⁻¹)	(kJ mol⁻¹)	(kJ mol⁻¹)	(10⁻³ kJ mol⁻¹ K⁻¹)	(protein/DNA)^c
1. G_psC_psTAATATATGC/GCAT_psA_psT_psA_psTTAGC	50	1.2 ± 0.2	−34.1 ± 0.4	−41.2 ± 2.8	−24 ± 10	0.79 ± 0.02
	100	0.51 ± 0.06	−32.0 ± 0.3	−38.9 ± 1.9	−23 ± 7	0.68 ± 0.02
	200	0.62 ± 0.12	−32.5 ± 0.5	−18.7 ± 1.2	47 ± 6	0.50 ± 0.07
2. GCTAATATATGC/GCATATATTAGC	50	0.78 ± 0.02	−33.1 ± 0.06	−32.3 ± 0.6	2.7 ± 1.7	0.95 ± 0.14
	100	0.54 ± 0.07	−32.1 ± 0.3	−18.9 ± 1.6	45 ± 6	1.07 ± 0.10
	200	NA^d	NA^d	NA^d	NA^d	NA^d

^aSequences of the two complementary strands are shown in the 5′ → 3′ direction. The recognition motif TAATA/TATTA is underlined. The positions for the phosphorothioate modification are indicated with ‘ps’.

^bFor the concentrations of Na⁺, 50 mM from the sodium phosphate buffer should be added to the values.

^cAverage values and standard deviations from triplicate experiments are shown.

^dHeat release was too small to be analyzed.