Figure 2.

Accuracy and precision using synthetic double-stranded control oligos. (A) Design schematic of control oligos – one blunt end, an identifiable 50 base pair UEI core, and an overhang of specific length and type on either end (purple rectangles). (B) Across replicate libraries, accuracy in overhang determination is highest when only overhanging UEIs are only considered when in reverse reads (includes blunt end UEIs from both reads), (C) Precision of UEI/control oligo combinations by UEI type. Columns are control oligo overhangs. Rows are UEI adapter overhangs. Asterisks show correct combinations; error bars show one standard deviation across libraries. (D) Base composition of overhangs by overhang type and length. Only overhangs for correct UEI/control oligo combinations are considered; proportions are means across libraries.