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. 2020 Feb 29;48(8):4480–4491. doi: 10.1093/nar/gkaa127

Figure 6.

Figure 6.

Knockdown of HCP5 reduced YB1 binding to MSH5 promoter region and inhibited the transcriptional activation of MSH5. (A) ChIP analyses of KGN cells were performed with IgG or YB1 antibody. ChIP products were amplified by PCR with specific primers of the MSH5 promoter region and subjected to electrophoresis analyses. (B) ChIP analyses of KGN cells were performed with IgG or YB1 antibody after HCP5 silencing, followed by qPCR in the MSH5 promoter region. Values of qPCR were obtained from triplicates and expressed as the mean ± SD (n = 3). *P < 0.05. Two-tailed Student's t-test. (C) ChIP analyses of KGN cells were performed with IgG or RNA pol II antibody after HCP5 silencing, followed by qPCR in the MSH5 promoter region. Values of qPCR were obtained from triplicates and expressed as the mean ± SD (n = 3). **P < 0.01. Two-tailed Student's t-test. (D) Schematic summary of the critical role of HCP5 in MSH5 expression and GCs function through direct binding and modulating the subcellular localization of YB1.