Figure 2.

B3H assay detects ProQ’s interaction with multiple RNA substrates. Results of B3H assays between a panel of RNA substrates with (A) wild-type ProQ (B) an R80A variant or (C) wild-type E. coli Hfq. β-galactosidase assays were performed with Δhfq reporter strain cells containing three compatible plasmids: one that encoded λCI or the CI-MS2^CP fusion protein, another that encoded α or an α-fusion protein (α-ProQ^ΔCTD, either with wild type ProQ or an R80A mutant or α-Hfq), and a third that encoded a hybrid RNA (a single MS2^hp moiety fused to cspE 3′ UTR, SibB, fbaA 3′ UTR, RyjB, ArcZ, ChiX, MgrR, OxyS, trpA terminator (T_trpA) or an RNA that contained only the MS2^hp moiety. The cells were grown in the presence of 0.2% arabinose and 50 μM IPTG (see Materials and Methods). Absolute β-gal values of a representative dataset are shown in Supplementary Figure S5A.