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. 2020 Feb 27;48(8):e45. doi: 10.1093/nar/gkaa116

Table 2.

Sequences of oligonucleotides used in the assays

Name	Sequence (5′→3′)
NDP-1 primer	`CCGCCTCCACCGCC`
FAM-dTTP probe	6-FAM/ `AGGACCGAG` /ZEN/ `GCAAGAGCGAGCGA` /IBFQ
FAM-dATP probe	6-FAM/ `TGGTCCGTG` /ZEN/ `GCTTGTGCGTGCGT` /IBFQ
FAM-dGTP probe	6-FAM/ `ACCATTCAC` /ZEN/ `CTCACACTCACTCC` /IBFQ
FAM-dCTP probe	6-FAM/ `AGGATTGAG` /ZEN/ `GTAAGAGTGAGTGG` /IBFQ
dTTP-DT1 template	`TCGCTCGCTCTTGCCTCGGTCCTTT` A `TTTGGCGGTGGAGGCGG`
dTTP-DT2 template	`TCGCTCGCTCTTGCCTCGGTCCTTT` A `TTT` A `TTTGGCGGTGGAGGCGG`
dATP-DT1 template	`ACGCACGCACAAGCCACGGACCAAA` T `AAAGGCGGTGGAGGCGG`
dCTP-DT1 template	`CCACTCACTCTTACCTCAATCCTTT` G `TTTGGCGGTGGAGGCGG`
dCTP-DT2 template	`CCACTCACTCTTACCTCAATCCTTT` G `TTT` G `TTTGGCGGTGGAGGCGG`
dGTP-DT1 template	`GGAGTGAGTGTGAGGTGAATGGTTT` C `TTTGGCGGTGGAGGCGG`
dGTP-DT2 template	`GGAGTGAGTGTGAGGTGAATGGTTT` C `TTT` C `TTTGGCGGTGGAGGCGG`