Table 1.

Equilibrium dissociation constants (K_ds) and other thermodynamic parameters derived for interactions between RNA (single stranded RNA and G-quadruplexes formed by TERRA RNA repeats) and proteins (UP1, UP1+RGG and the isolated RGG-box) using ITC experiments

S.No.	Experiment	K d (μM)	ΔG (kcal/mol)	ΔH (kcal/mol)	TΔS (kcal/mol)	n
1	UP1:ssRNA-18	2.37±0.49	−7.67±1.58	−19.7±1.23	−12.03±2.81	1.04±0.05
2	UP1+RGG:ssRNA-18	2.28±0.26	−7.69±0.86	−20.4±0.71	−12.71±1.57	0.97±0.03
3	UP1:TERRA-6	2.02±0.17	−7.76±0.64	−31.31±1.4	−23.55±2.04	1.22±0.04
4	UP1+RGG:TERRA-6	2.38±0.23	−7.66±0.75	−38.84±2.74	−31.18±3.48	1.14±0.07
5	UP1:TERRA-12	2.04±0.47	−7.75±1.77	−36.38±5.61	−28.63±7.38	0.93±0.12
6	UP1+RGG:TERRA-12	1.07±0.14	−8.13±1.08	−43.01±2.48	−34.88±3.56	0.88±0.04
7	UP1:TERRA-24	1.71±0.17	−7.86±0.76	−29.66±0.95	−37.52±1.70	0.80±0.02
8	UP1+RGG:TERRA-24	0.92±0.17	−8.23±1.53	−41.88±2.81	−33.65±4.34	0.82±0.04
9	UP1+RGG:TERRA-24 (with 20% PEG 200)	3.0±0.55	−7.53±1.40	−40.89±4.15	−33.36±5.55	1.14±0.09
10	RGG:TERRA-24	9.0±4.3	−6.88±3.31	−0.67±0.27	6.21±3.58	1.06±0.34