Table 1.
Isothermal titration calorimetry (ITC) measurements
| Protein | RNA | K d (μM) | ΔH (kcal/mol) | ΔS (cal/mol·K) |
|---|---|---|---|---|
| mRBM20(513–621) | AUCUUA | 5.7 ± 0.2 | −19.7 ± 0.5 | −42 ± 2 |
| mRBM20(513–621) | GUCUUA | 4.0 ± 0.1 | −21.0 ± 0.5 | −46 ± 2 |
| mRBM20(513–621) | ACCUUA | 13 ± 2 | −17 ± 1 | −36 ± 4 |
| mRBM20(513–621) | AUUUUA | 140 ± 30 | −10 ± 1 | −18 ± 4 |
| mRBM20(513–621) | AUCCUA | 210 ± 3 | −9 ± 2 | −14 ± 8 |
| mRBM20(513–621) | AUCUCA | 95 ± 1 | −27.0 ± 0.2 | −72 ± 1 |
| mRBM20(513–621) | AUCUUG | 4.8 ± 0.1 | −18.8 ± 0.4 | −39 ± 2 |
| mRBM20(513–621)H523A | AUCUUA | 67 ± 12 | −37 ± 4 | −104 ± 15 |
| mRBM20(513–621)N526A | AUCUUA | 9 ± 1 | −19 ± 1 | −40 ± 5 |
| mRBM20(513–621)V537I | AUCUUA | 10.3 ± 0.7 | −18.6 ± 0.4 | −40 ± 1 |
| mRBM20(513–621)Q558A | AUCUUA | 3.4 ± 0.7 | −17.4 ± 0.4 | −34 ± 2 |
| mRBM20(513–621)F560A | AUCUUA | 156 ± 6 | −29 ± 2 | −80 ± 5 |
| mRBM20(513–621)Q577A | AUCUUA | 6.6 ± 0.8 | −22 ± 1 | −50 ± 3 |
| mRBM20(513–621)R591M | AUCUUA | 12.4 ± 0.6 | −22.3 ± 0.34 | −52 ± 1 |
| mRBM20(513–621)R595M | AUCUUA | 29.3 ± 0.3 | −29.1 ± 0.8 | −77 ± 3 |
| mRBM20(513–621)Y596A | AUCUUA | 74 ± 13 | −6 ± 1 | 0 ± 5 |
| mRBM20(513–609)Δα3 | AUCUUA | 43 ± 1 | −16.2 ± 0.2 | −35 ± 1 |
| mRBM20(513–649)+RS | AUCUUA | 4.3 ± 0.4 | −17 ± 2 | −33 ± 7 |
| mRBM20(513–621) | vIRES-SL a | 8 ± 1 | −6.7 ± 0.6 | 1 ± 2 |
a GGGACCUGGUCUUUCCAGGUCCC (derived from PDB ID 2N3O in which the underlined sequence base-pairs to form the stem).