Table 2.

NMR and refinement statistics for the RNA-bound and unbound RBM20_(513–621)

	AUCUUA bound mRBM20	Unbound mRBM20
Number of structures	25	25
Distance constraints
Total	3693	2838
Protein intraresidue	929	823
Protein interresidue
Sequential (|i – j| = 1)	442	385
Short range (1 < |i – j| < 4)	199	177
Medium range (3 < |i – j| < 6)	79	76
Long range (|i – j| > 5)	398	336
RNA	104	–
Intermolecular	69	–
Ambiguousa	1445	1041
Hydrogen bonds	28	–
Total dihedral angle constraints
Protein	254	224
RNA	28	–
Residual dipolar couplings
1DHN	82	–
Structure statistics
Violations (mean and SD)
Distance constraints (Å)b	0.018 ± 0.000	0.020 ± 0.001
Dihedral angle constraints (°)c	1.48 ± 0.06	1.40 ± 0.07
Residual dipolar couplings, Qd	0.24 ± 0.01	–
Deviations from idealized geometry
Bond lengths (Å)	0.004 ± 0.000	0.004 ± 0.000
Bond angles (°)	0.54 ± 0.01	0.51 ± 0.02
Impropers (°)	1.41 ± 0.04	1.5 ± 0.1
Ramachandran plot (%) e
Most favoured	93.5 (88.4)	95.6 (82.1)
Additionally favoured	6.3 (10.1)	4.4 (14.5)
Generally allowed	0.2 (0.6)	0.0 (1.8)
Disallowed	0.0 (0.8)	0.0 (1.5)
Average pairwise rmsd (Å)
Protein backbone 2° structure	0.25 ± 0.04	0.20 ± 0.04
Protein heavy 2° structure	0.56 ± 0.06	0.56 ± 0.07
Protein backbone all	1.5 ± 0.2	3.2 ± 0.8
Protein heavy all	1.9 ± 0.2	3.6 ± 0.8

^aA standard class of distance restraints in Aria2.3, derived from cases in which the peak volume is contributed by two or more overlapping NOE crosspeaks

^bNo violations >0.5 Å.

^cNo violations >10°.

^dCalculated for each structure in the ensemble using the method described by (73).

^eDetermined by using PROCHECK-NMR (74). Main values display hetNOE > 0.5 and include residues 520–617 for the bound form, and 520–598 for the unbound form. Values that include all residues are in parentheses.