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. 2020 Mar 18;48(8):4256–4273. doi: 10.1093/nar/gkaa174

Figure 3.

Figure 3.

Heat stress induces expression of spermatogenesis-enriched genes in adult hermaphrodites. (A) Comparison of expression changes in wild-type animals cultured at 25°C compared to wild-type animals cultured at 20°C for published enriched gene sets. Notches indicate the 95% confidence interval of the median; black line indicates median. n.s. denotes not significant and indicates a P-value > 0.05, * indicates a P-value ≤ 0.05, **** indicates a P-value ≤ 0.0001. (B) qRT-PCR of spermatogenesis factors in hermaphrodites of wild-type and mut-16 mutant animals cultured at 20°C and 25°C. Expression is normalized to rpl-32 and relative to expression in wild-type animals cultured at 20°C. Error bars indicate standard deviation. (C) qRT-PCR of spermatogenesis factors in the germlines of hermaphrodites of wild-type and mut-16 mutant animals cultured at 20°C and 25°C. Expression is normalized to cdc-42 and relative to expression in wild-type animals cultured at 20°C. Error bars indicate standard deviation.