Figure 1.

Conjugation of ASO with palmitic acid potentiates target mRNA knockdown in several tissues. A 3–10–3 PS cEt ASO (GCATTCTAATAGCAGC) targeted to MALAT1 or the same ASO conjugated at the 5′ terminus with palmitic acid was administered subcutaneously to male C57Bl/6 mice at concentrations ranging from 0.3 to 30 μmol/kg. Mice were terminated 72 h after administration and MALAT1 mRNA was extracted from liver, heart and quadricep tissues and quantified by qPCR. The ED50 is lowered 3–4-fold in heart and quadriceps.