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. 2020 Mar 23;48(8):3999–4012. doi: 10.1093/nar/gkaa172

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Extranuclear expression of sqADAR2 in squid tissue. (A) A schematic of squid anatomy showing the locations of tissue samples used in this study. OL = optic lobe of the CNS; GI = gill; HE = heart (branchial); SG = stellate ganglion of the PNS (note that the GFL is a part of the SG); EP = epithelium; GFL = giant fiber lobe; SN = small nerves (small diameter axons that form a mesh around the GA); GA = giant axon; AP = axoplasm. (B) Western blot analysis of nervous and non-nervous tissues extracted from D. pealeii and probed with α-sqADAR2 antibody (top panel). Blocked control is also shown (bottom panel). Purified sqADAR2 proteins (2a and 2b) were used as positive controls. (C) Western blot analysis of homogenates from different neuronal tissues extracted from D. pealeii and probed with α-sqADAR2 antibody. (D) Western blot analysis of whole somata samples from the squid SG after total, cytoplasmic and nuclear fractionation. Images were taken using a LI-COR odyssey infrared imaging system; n = 2. sqADAR2a (2a) is 89 kDa, sqADAR2b is 71 kDa, histone is 18 kDa and tubulin is 50 kDa. GI = gill; OL = optic lobe; HE = heart; SG = stellate ganglion; EP = skin epithelium; GA = giant axon; GFL = giant fiber lobe; AP = axoplasm; SN = small nerve fibers; 2a = sqADAR2a; 2b = sqADAR2b.