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. 2020 Mar 23;48(8):3999–4012. doi: 10.1093/nar/gkaa172

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Axoplasm has A→I conversion activity. (A) Images of thin-layer chromatography plates used to separate radiolabeled adenosine and inosine from dsRNA editing assays with different tissues extracted from D. pealeii. Recombinant sqADAR2b was incubated as a positive control and assays with water were performed as negative controls. (B) Editing percentages were quantified based on A-to-I conversion; n = 3 ± standard error of the mean (SEM). AD = sqADAR2b; OL = optic lobe; SG = stellate ganglion; SN = small nerve fibers; GA = giant axon; AP = axoplasm; HE = heart. OL, SG and HE samples contain both nuclei and cytoplasm.