Figure 5.
Axoplasm has A→I conversion activity. (A) Images of thin-layer chromatography plates used to separate radiolabeled adenosine and inosine from dsRNA editing assays with different tissues extracted from D. pealeii. Recombinant sqADAR2b was incubated as a positive control and assays with water were performed as negative controls. (B) Editing percentages were quantified based on A-to-I conversion; n = 3 ± standard error of the mean (SEM). AD = sqADAR2b; OL = optic lobe; SG = stellate ganglion; SN = small nerve fibers; GA = giant axon; AP = axoplasm; HE = heart. OL, SG and HE samples contain both nuclei and cytoplasm.