Figure 1.

Selection scheme of ssODNs that bind to VCaP exosomes but not to LNCaP exosomes. (A) A highly diverse library of 10¹¹ ssODNs was subjected to five rounds of positive selection. (B) Schematic for the negative selection cycle introduced at round 2 for the remaining selection. After mixing of exosomes with ODN library the unbound DNA was removed by precipitating exosomes and bound ssODNs by polymer. (C) Summary of the succession of selection rounds against exosomes from VCaP and LNCaP prostate cancer cell lines.