Table 1.
List of proteins identified in VCaP cell exosomes after pull-downs with Sequence 7a
| Protein | Description | Significance | WB verified | Reference |
|---|---|---|---|---|
| CHMP1B | Charged multivesicular body protein 1b | Part of ESCRT machinery that plays role in exosome biogenesis. Identified in exosomes from expressed prostatic secretions | Yes | (60–64) |
| CHMP2A | No | |||
| CHMP4B | Charged multivesicular body protein 4b | Yes | ||
| CHMP4C | Charged multivesicular body protein 4c | Yes | ||
| MVB12A | Multivesicular body subunit 12A | Yes | ||
| SH3GL1 | Endophilin-A2 | No | ||
| VPS28 | Vacuolar protein sorting-associated protein 28 homolog | Yes | ||
| Syntenin-1 | Syntenin-1 | Associated to ESCRT machinery that plays role in exosome and prostasome biogenesis | Yes | (60,62,63,65) |
| YBX1 | Nuclease-sensitive element-binding protein 1 | Connected to ESCRT-1 complex by interacting with TSG101 Marker in CRPC and role in resistance to enzalutamide | Yes | (46) |
| RPLP1 | 60S acidic ribosomal protein P1 | No | ||
| RBM3 | RNA-binding protein 3 | No | ||
| RPL30 | 60S ribosomal protein L30 | No | ||
| HBA2 | Hemoglobin subunit alpha | No | ||
| S100A8 | Protein S100-A8 | No | ||
| CXCL11 | C-X-C motif chemokine 11 | Increased gene expression in prostate cancer | No | (28) |
| DNAJA2 | DnaJ heat shock protein family (Hsp40) member A2 | Upregulated in spermatocytes; close homolog of DNAJA1, which is involved in spermatogenesis and AR signaling | Yes | (48,49) |
| PTBP1 | Polypyrimidine tract-binding protein 1, hnRNP-1 | Cancer associated splicing factor. Known association with breast tumorigenesis; required for tumor cell growth | Yes | (66) |
| eEF1A2 | Elongation factor 1-alpha 2 | Promotes proliferation and inhibits apoptosis in prostate cancer | Yes | (67) |
| LAMA3 | Laminin subunit alpha-3 | Yes | ||
| CPSF5/ NUDT21 | Cleavage and polyadenylation specificity factor subunit 5 | Yes | ||
| CPSF7 | Cleavage and polyadenylation specificity factor subunit 7 | No |
aAdditional MS data are listed in Supplementary Table S2.