Table 1.
Primers used for real-time qPCR assays.
| Symbol | Gene Name | Forward Primer | Reverse Primer | Accession number | Amplicon length (bp) |
Source |
|---|---|---|---|---|---|---|
| RBL2 | RB Transcriptional Corepressor Like 2 | AGAGATCCAACCCAGGCTA | TTGTTCCTGCTCAATAACAGAT | NM_001098073.1 | 176 | Bos Taurus |
| CCND1 | Cyclin D1 | ACTACCTGGACCGCTTCCT | TTGGAGAGGAAGTGCTCGAT | NM_053056.2 | 253 | Homo Sapiens |
| CCNA2 | Cyclin A2 | CCTGCAAACTGCAAAGTTGAA | GGTGAAGGTCCAGGAGACA | NM_001237.2 | 216 | Homo Sapiens |
| CDK2 | Cyclin Dependent Kinase 2 | GGTGTACCCAGTACTGCCA | CGCAGAGGCATCCATGAATT | BT020790.1 | 158 | Bos taurus |
| CDK4 | Cyclin Dependent Kinase 4 | ATTTCCTTCATGCCAACTGCA | CCAACACTCCACATGTCCAC | NM_000075.2 | 216 | Homo Sapiens |
| CDK6 | Cyclin Dependent Kinase 6 | GCATCGTGATCTAAAACCACA | GAGTCCAATCACGTCCAAGA | NM_001259.5 | 285 | Homo Sapiens |
| BCL2 | Apoptosis Regulator Bcl-2 | GGAGCTGTATGGCCCTAGCA | TGAGCAGTGCCTTCAGAGACA | NM_001166486 | 68 | Bos Taurus |
| BAD | BCL2 Associated Agonist Cell Death | CGATATGGCCGCGAACTC | GCTCTTCGGGCGAGGAA | NM_001035459 | 75 | Bos Taurus |
| APAF1 | Apoptotic Peptidase Activating Factor 1 | TTCACTCCAGTGGCCTGTTG | CAAAGCCACCACTGCCAAAT | NM_001191507 | 101 | Bos Taurus |
| CASP3 | Caspase 3 | CCATGGTGAAGAAGGAATCATTT | CCCCTCTGAAGAAACTTGCTAATT | AF068837 | 77 | Ovis aries |
| CASP8 | Caspase 8 | TGGCTGCCCTCAAGTTCCT | GGAATAGCATCAAGGCATCCTT | NM_001045970 | 81 | Bos Taurus |
| CASP9 | Caspase 9 | CAGGTTGCCTCGCTTTGG | CTCGATCATGTCGGGAGTGA | NM_001205504 | 63 | Bos Taurus |
| CDKN1A | CDK Inhibitor 1A | GAGGACCACTTGGACCTGT | TCTGCGTTTGGAGTGGTAGA | NM_001098958.1 | 146 | Bos taurus |
| CDKN1B | CDK Inhibitor 1B | GCTTGCCCGAGTTCTACTAC | CATTTTCTTCTGTTCTGTTGG C | NM_004064.2 | 273 | Homo Sapiens |
| RB1 | RB Transcriptional Corepressor 1 | CATGCGCTCTTGAGGTTGTA | GGTTGCTTGTGCCTCTGAAT | NM_000321.1 | 436 | Homo Sapiens |
| TP53 | Tumor Protein 53 | ACGACCGGAACACCTTTAGACACA | ACCACGAGAGTCTTCCAGTGTGAT | FJ855223.1 | 166 | Ovis aries |
| MSTN | Myostatin | ACGATGTCCAGAGAGATGACAGCA | ATCAGACTCCGTGGGCATGGTAAT | NM_001009428.1 | 97 | Ovis aries |
| FST | Follistatin | TGCACTCCTCAAGGCCAGATGTAA | ATTAGTCTGGTCCACCACGCATGT | NM_175801.2 | 126 | Bos taurus |
| FNDC5 | Irisin | CCAGTGAACGTCACCGTCA | CCTGGATGAAGCGCAGCAT | BC149873.1 | 135 | Bos taurus |
| RPL37A | Ribosomal Protein L37a | ACCAAGAAGGTCGGAATCGT | GGCACCACCAGCTACTGTTT | XM_027965159 | 192 | Ovis aries |