Figure 2. Effect of AICAR on GLTε transcription, surface IgE expression, and IgE production by anti-CD40-stimulated B cells. Purified mouse B cells (1×10⁶ cells/ml) were stimulated with IL-4 (1 or 2.5 or 5 ng/ml) and AICAR (200 µM) in the presence of anti-CD40 (2 µg/ml). (A) After 2.5 days of culture, RNAs were isolated and the levels of GLTs and AID mRNA were measured by RT-PCR. (B) After 4 days of culture, cells were stained with anti-IgE-FITC and anti-B220-PE, and surface Ig expression was analyzed by flow cytometry. (C) After 7 days of culture, supernatants were harvested, and the levels of Ig production were measured by isotype-specific ELISA. Data presented are the means±SEM from three independent experiments. (D) After 4 days of culture, cells were stained with anti-CD69-FITC and B cell activation was analyzed by flow cytometry.

NS, not significant.

^*p<0.05.