Figure 3. Effects of Sesn2 and AICAR on the expression of surface IgE and the activation of STAT6 and AMPK. (A) Purified B cells (1×106 cells/ml) from Sesn2+/+ or Sesn2−/− mice were stimulated with IL-4 (2.5 ng/ml) and AICAR (200 µM) in the presence of anti-CD40 (2 µg/ml). After 4 days of culture, cells were stained with anti-IgE-FITC and anti-B220-PE, and surface Ig expression was analyzed by flow cytometry. (B) Purified B cells (5×106 cells/ml) from Sesn2+/+ or Sesn2−/− mice were stimulated with IL-4 (100 ng/ml) for 30 min in the presence of anti-CD40 (2 µg/ml). Cells were harvested to measure expression of the indicated proteins.
