Fig. 3.

SKP2 expression is upregulated in vivo in the Notch-induced T-ALL model. Lin⁻ cells from C57Bl/6 mice (CD45.2) were transduced with MSCV-GFP (Vector) or MSCV-ICN1-GFP (ICN) constructs. 2.5 × 10⁴ ICN/GFP⁺ cells were transplanted into lethally irradiated BoyJ (CD45.1). Recipients were analyzed for: a Fold change for human Skp2 mRNA expression measured by qRT-PCR in the BM (left panel; n = 5) and Sp (right panel; n = 6 (vector) and n = 10(ICN)). b CHIP assay. DNA isolated from the thymus of mice transplanted with ICN-GFP⁺ cells was immunoprecipitated with anti-Notch1 (N1) or anti-Jagged1 (irrelevant) antibodies Top panel: Scatter graph shows fold changes in SKP2 promoter enrichment measured by qRT-PCR (each group n = 3). Bottom panel: schematic representation of the Notch/RBPj binding sites within the mouse Skp2 promoter. All results are from two independent experiments. In scatter plots average is shown by a horizontal line.*p < 0.05, **p < 0.01, ***p < 0.005 by t-test