Fig. 2.

Enhanced UPS activity is observed in the intestine but not in the body wall muscle at 25 °C. Fluorescent micrographs and quantification of UbG76V-Dendra2 degradation in a intestinal and b body wall muscle cells at both 20 °C and 25 °C. Graph columns represent the average relative percentage of fluorescence prior to (green) or after (red) photoconversion, and are the average of a minimum of five independent experiments. Number of animals imaged is listed in Supplementary Table 1. Error bar, SEM; **p < 0.01; ns, not significant. Scale bar, 500 μm