Fig. 3.

Proteasome catalytic activity and amount of 20S proteasome are unchanged in whole animal lysates of animals exposed to 25 °C. a In-gel proteasome activity assay (upper image), Coomassie staining of same gel (lower image) and quantification (right). b Western blot (left) and quantification (right) against proteasomal 20S alpha subunits. Anti-alpha-tubulin antibody was used as a normalisation control. Quantification graphs show the average fold change compared with 20 °C (set to 1) and are the average of 10 independent experiments. RP, regulatory particle; CP, core particle. Error bar, SEM; ns, not significant