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. 2020 Apr 30;5:8. doi: 10.1038/s41536-020-0093-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Schematic overview of all established kidney organoid protocols starting from hPSCs. The timeline shown on top indicates the number of days the protocol takes to induce the developmental stages in vitro. Color-coded boxes are not unique, but help visualize the different key steps in the respective protocols. Note that the protocol described here by Taguchi, 2017 highlights the differentiation process to ureteric bud cells and is an addition to their previous protocol (Taguchi, 2014) that exclusively induces renal progenitor cells.