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. 2020 Apr 30;10:7340. doi: 10.1038/s41598-020-64226-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alteration in the metabolites following VDR Deletion and HFD intervention: (A) Random forest (RF) analysis showing the top 30 metabolites responsible for classification as mean decrease accuracy values. Most vital biochemicals are listed from data derived from fecal samples of VDR^LoxP, VDR^∆IEC mice fed with HFD or chow diet. Different colors indicate different biochemicals like, light blue = amino acid, purple = co-factors &vitamins, blue = lipids, orange = nucleotide, red = peptide. (B) Principal Component Analysis (PCA) on metabolite level Correlations. Color code: light blue= VDR^LoxP + chow, orange = VDR^LoxP + HFD, purple= VDR^∆IEC chow, red = VDR^∆IEC + HFD. VDR^ΔIEC group (Chow diet: N = 17; HFD: N = 7), & VDR^LoxP (Chow Diet: N = 16; HFD: N = 6).